16S rDNA High Throughput Sequencing Bacterial Diversity Analysis of Cow Mastitis

Background: Bacterial mastitis in cows is a huge challenge to the dairy cattle breeding and production industry. This study examined relationship between bacterial diversity at different sampling sites farms. Methods: High-throughput sequencing of 16S rRNA V4 region was used analyze abundance population sampled from seven farm, named healthy cow milk (N), (C), perimammary (T), collecting cup (B), turntable surface (P), feces (F) bedding material (W). Result: In total, 2757356 reads were obtained results, which annotated 29 phyla, 67 classes, 166 orders, 295 families, 696 genera 306 species. all samples, Proteobacteria Firmicutes dominant microflora, while Oscillospira-UCG-005 Atopostipes proportions, indicating certain differences flora structure among samples. Furthermore, alpha analysis revealed high richness